■ 基本信息
啟動(dòng)子: | CaMV 35S promoter |
復(fù)制子: | pVS1V |
終止子: | NOS |
質(zhì)粒大小: | 12361bp |
原核抗性: | Kan |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物 |
5'測(cè)序引物: | M13F:TGTAAAACGACGGCCAGT |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: | Kan
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原核抗性: | Hyg |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the mgfp5:gusA fusion reporter genes.The pCambia vector backbone is derived from the pPZP vectors. The pCambia1304 vector offers:1.high copy number in E.coli for high DNA yield?2.pVS1 replicon for high stability in Agrobacterium 3.small size?4.restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest 5.bacterial selection with kanamycin?6.plant selection with hygromycin B 7.simple means to construct translational fusions to mgfp5-gusA reporter genes.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK2374pCAMBIA1304質(zhì)粒.txt
