■ 基本信息
啟動(dòng)子: | H1 promoter |
復(fù)制子: | pBR322 ori，SV40 ori |
質(zhì)粒大小: | 4555bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 基因沉默 |
片段類型: | shRNA
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Hyg
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pSilencer3.1-H1-Stuffer-Hygro是一個(gè)哺乳細(xì)胞干擾載體，通過BamHI和HindIII酶切位點(diǎn)可連入SiRNA，點(diǎn)擊查看本質(zhì)粒的參考資料：
pSilencer hygro Kit.pdf.（點(diǎn)擊查看）
The pSilencer vectors employ RNA polymerase III (pol III) promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1717 pSilencer3.1-H1-Stuffer-Hygro哺乳干擾質(zhì)粒.txt
