■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細胞，哺乳細胞雙雜交載體 |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 雜交 |
片段類型: |
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: |
|
■ 質(zhì)粒簡介
The protein product of the Id cDNA sequence in the pBIND-Id Vector is known to interact with the protein product of the MyoD cDNA sequence in the pACT-MyoD Vector.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
GGCTCGTTTAGTGACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCA
CAGTTAAATTGCTAACGCAGTCAGTGCTTCTGACACGACAGTCTCGAACTTAA
GCTGCAGTGACTCTCTTAAGGTAGCCTTGCAGAAGTTGGTCGTGAGGCACTGG
GCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAAAAACTGG
GCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTT
ACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTA
CAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCCAGCTTG
AAGCAAGCCTCCTGAAAGATGAAGCTACTGTCTTCTATCGAACAAGCATGCGA
TATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCA
AGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCT
CCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGA
ACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAA
TGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATAT
GCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGA
GTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGAATTCCCGGGGATC
CGTCGACATAAATTCCCACTTGGTCTGTCAGAGCAAAGCGTGGCCATCTCGCG
CTGCGCTGGGACGCGCCTGCCCGCCTTGCTGGACGAGCAGCAGTGAACGTCCT
GCTCTACGACATGAACGGCTGCTACTCACGCCTCAAGGAGCTGGTG
