■ 基本信息
啟動子: | SV40 |
復制子: | pUC ori |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，信號通路報告載體 |
質粒大小: | 4641bp |
原核抗性: | Amp |
克隆菌株: | 大腸桿菌DH5α |
培養(yǎng)條件: | 37℃，有氧 LB |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'測序引物: | 根據序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: | Promoter |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | Fluc
|
■ 質粒簡介
The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the bla (β-lactamase or Ampr) gene, have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK474pGL4.13[luc2-SV40]哺乳報告質粒.txt
